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The Oct4 protein (360 aa) has two DNA-binding domains, POUS (85 aa, orange) and POUHD (51 aa, red) connected by a linker of 15 aa. (B) The DNA-binding complex structure (Upper) and sequence motif (Lower) for the Sox2 HMG domain. The complex structure is based on PDB entry 1GT0.

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We first performed MD simulations of a single Oct4 binding to the LIN28B nucleosome. As the initial structure of these simulations, we used the nucleosome at the u=104 position and put Oct4 at different positions around the nucleosome.

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Nucleosome structures are flexible enough to be regulated by other factors. The spontaneous nucleosome dynamics such as partial unwrapping and rotation-coupled sliding of the nucleosomal DNA has been well documented by both experiments and MD simulations (13, 15⇓–17). The partial unwrapping of DNA opens up more space for TF to bind (13), whereas TF binding, in turn, changes the accessibility of deeper nucleosomal DNA for additional TF binding (21). These regulations can be considered as allosteric regulation (12). Here our simulation results provided clues for another possible allosteric mechanism of collaborative TF−nucleosome binding.

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It’s certainly possible, but the complexity of the operation will depend on what system you’ve currently got versus the system you want. As you’d expect, it’s much easier to go from tank to tankless by using the same power source, either electricity or gas. Switching between the two requires a significant overhaul that will set you back stacks of cash.

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Before putting the TFs onto nucleosomes, we first studied the binding of Sox2 and Oct4 to naked double-stranded DNAs (dsDNAs). The DNA sequences used for Sox2 and Oct4 binding are both taken from the Hoxb1 regulatory element and are the same as those used in previous NMR studies (37, 38) (Table 1).

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Compare this to a tank-based water heater, which heats a whole tank full of water before delivering it to your faucet. These older and less desirable systems take several minutes to warm enough water, and the hot water tank tends to cool down with intermittent use. As a result, although they may be cheaper, tank-based systems fail to provide a consistent supply of hot water when you need it and use much more energy.

We then turned to simulate the binding of Sox2 onto the LIN28B nucleosome, starting from the highest population u=104 position. As clarified above, the rotational position of the Sox2 target motif determines the binding pattern and position of Sox2.

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On the downside—there’s always a downside—gas is a finite resource that can’t be harnessed from the sun or your engine while you drive. You’ll have to fill your propane/LPG tanks manually, which costs money and can be a non-option when there’s no major town within a hundred-mile drive. Worse still, you could run out of gas the moment you hop in the shower or turn on your stove. In that case, you would have to do without until you can find someone to refill your tank.

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The DNA binding position bSox2 (see Methods for definition) of Sox2 (purple dots in Middle) and the bending angle η of DNA (see SI Appendix, Supplementary Methods for definition; gray dots in Lower) are plotted as functions of MD steps. The cyan region in Middle (DNA indices 7 to 13) represents the consensus sequence for Sox2 binding.

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If that’s you, then grabbing the low-powered Ecosmart POU will save you a ton of cash. The meager 0/5 GPM flow rate means the unit isn’t powerful enough to deliver a full-flow shower, but it’s easily sufficient for the sink. Its low amperage draw and wattage rating saves money upfront and drains your RV house batteries way less than a more powerful system (off-grid camping won’t be a problem with this bad boy). The compact unit even has a handy digital display so you can set the temperature.

One of the most critical features of the nucleosome as a TF blocker is the rotational phasing of the nucleosomal DNA (43⇓–45). The pattern of DNA wrapping around the histones introduces a 10-bp periodicity of DNA being buried or exposed in nucleosomes. As revealed by previous experiments, the intrinsic phasing of DNA, and the rotational position of the TF target motif, play essential roles in determining the TF binding sites (7, 27, 43).

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Granted, it’s not going to compete with a dedicated hot water system. Still, the cost-effective solution is simple to set up and among the best performing portable options out there. And unlike other portable heaters, the Camplux has a shut-off protection system for when oxygen levels drop too low, thus winning it top marks for safety. Do remember, though, that this and other portable systems are strictly for outdoor use only (CO2 output isn’t something to mess around with).

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This high-performance model maxes out at 9,000 W, which is way too much to use off-grid. If you’re looking for an infrared tankless water heater you can use with a solar system or generator, consider the more modest 3,400 W or 6,000 W model instead.

Before we get stuck into our top ten list, there are several things you need to consider. The best RV tankless water heater for you ultimately depends on your budget, heating requirements, and existing RV setup. Take the following considerations into account before hitting the ‘buy now’ button.

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With a tank-based water system, you’ll stand around waiting for the water to heat up as gallons of the good stuff flow wastefully down the drain. With a tankless hot water system, however, you can jump straight into the shower within moments of turning it on and enjoy piping hot water in an instant. No more wasting water while you wait for it to become hot enough to wash in.

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Pumping out a whopping 55,000 BTUs, the RV-550 is among the best performing RV heaters on the market. Expect endless streams of piping hot water to finally become the norm—you can even run two hot showers at the same time with that kind of output.

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Enjoying a long hot shower is a blessing when you’re living in an RV, so it makes sense to invest in the best possible water heater you can afford. Tankless heaters provide piping hot water in an instant and never run out halfway through the deed.

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Our results suggest that Sox2 binding can regulate the accessibility of other TFs’ targets in the nucleosomal DNA. Sox2, together with the subsequently bound TFs, may interplay with the intrinsic free energy landscape of nucleosomes (53) and cause possible large-scale nucleosome conformational changes. Interestingly, the recently solved cryo-EM structures of nucleosome bound by Sox2/Sox11 and Sox2+Oct4 revealed sequence-dependent different pioneer TF−nucleosome binding mechanisms (28, 29). On a modified 601 nucleosome, Sox2 and Oct4 together recognized their consensus motif at SHL-6 and bent the entry/exit region of DNA away from histone (29), whereas, when Sox2 bound to its motif at SHL+5, the DNA conformational change was localized (29), consistent with our simulation results (SI Appendix, Fig.

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Apparently, the CG models we used here sacrificed atomistic details of interactions to gain sampling efficiency. Although our simulation results are consistent with experimental results (6, 7, 28, 29), a high-resolution picture of the physical interactions such as hydrogen bonds and salt bridges was not provided. To get such information, reconstruction of all-atom structures from CG models and all-atom simulations might be helpful. As an example, we rebuilt the all-atom structures for the POUS domain and the H2A−H2B dimer and performed simulations to check the stability of the structure of the POUS recognizing the acidic patch (SI Appendix, Fig.

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In normal circumstances, they use both resources simultaneously to heat water as it passes through the system. But should your batteries start running low, or your propane tank begins to dwindle, it switches over to the more abundant energy source so you can keep enjoying hot water for many days to come. Consequently, they’re the hot water system of choice for the intrepid RVer who loves boondocking in remote and wild locales.

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As you’d expect at this price range, the system comes with all the technological bells and whistles to make temperature control a breeze. The in-built VariFlame system automatically adjusts the gas output based on the water flow, so you won’t have to keep fiddling with the faucet to find the ideal temperature. It’s ultra-energy-efficient, too, which means fewer tiresome trips to the gas refiller to top up your propane tank. Northerners will be happy to hear the system can quickly and easily be winterized.

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Some of the connectors and adapters are prone to breaking, although these can be cheaply replaced at your local hardware store. Remember to drain the unit entirely before the outside temperature hits freezing, otherwise you can permanently break the heating element and have to pony up for a new one.

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But that’s where infrared technology comes in, which creates heat without water ever coming into contact with a metallic coil. This way, you won’t have to replace the element, saving serious coin on maintenance costs. Of course, all this newfangled technology results in a higher upfront investment than your standard electric heater. But if you plan to use it for many years to come, the system will work out cheaper in the long run, and you’ll enjoy lovely hot showers every time.

Once it’s set up, you won’t pay a cent because you’ll be using the energy from your alternator or your solar panels as you drive. And when you’re plugged into the shore power at the RV park, you won’t have to pay either because electricity is already included in your nightly rate.

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We found that the highly populated “ATTA” sites (at 15 to 18 and 39 to 42) and the canonical “GAAT” (at 74 to 77) in Oct4-naked DNA binding were no longer favored binding positions in the LIN28B nucleosome. Instead, the “CAAT” sequence piece (61 to 64) inside the Sox2 target motif became the most probable binding site.

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Stiebel Eltron has been producing superb single-point-of-use water heaters from their Berlin factory since the 1920s, so you can rest assured they’ve got it worked out—nothing but high-quality German engineering here. Their DHC model is perfect for intrepid nomads living out of their RV.

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Almost all users opt to set the unit up outside their RV, and the L10 goes from unpacked to ready to roll in a matter of minutes. Hang it up, connect it to your 20lb propane tank, connect the water outlet and inlet, and you’re ready to enjoy instant hot water in the wilderness. Granted, it’s a bit more involved to set up than a fixed heater, but getting things rolling is about as easy as it can be with a portable heater.

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Consequently, you should work out how many gallons per minute of hot water you and your whole traveling crew might use. For starters, most showers run between 1/5 and 3 gallons per minute. However, if someone else wants to do the dishes at the same time, you’d need to add another 1/5 GPM to heat the sink faucet as well. Keep in mind that you’re not only limited by the flow rate, but by the water storage capacity of the RV itself.

Tankless heaters are not only smaller, they’re also lighter. As you know, the less weight you’re hauling on your RV, the less fuel you’ll burn through. Optimizing your miles per gallon is a significant consideration of every budget-conscious RVer, and going with a lighter hot water system will save you money in the long run.

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Full-time RVers who frequent cold environs are constantly on the lookout for ways to obtain the holy grail of luxury mobile living: a long, steamy shower when it’s freezing out. And even though this pricey system from PrecisionTemp comes in at well over a grand, that’s money well spent for travelers tired of taking those frustratingly short navy showers.

You’ve probably noticed in our reviews that we use the coveted hot shower as the benchmark to determine whether a hot water system delivers the goods. But what if you’re not interested in hot showers at all? What if all you need is something to heat the sink water enough to scrub those stubborn ketchup stains off your endless pile of dirty dishes?

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The RV lifestyle is hard to beat—what could be better than cruising around the country in a decked-out mobile home while exploring remote wilderness regions of unfathomable beauty? But life on the road isn’t always luxurious: no matter how you spin it, you’re still camping. Yet for those with the means to invest, there are ways to make the RVing experience more comfortable, lighter weight, and energy-efficient. And one of the most comforting things to have on a road trip is an unlimited supply of piping hot water.

We first calibrated parameters in the PWMcos model for Sox2 and Oct4 by matching their simulated dissociation constant (Kd) with DNA to the experimental results (37, 38). These simulations were carried out at the ionic concentration of 150 mM, which was also used in experiments. We monitored the distance between the COM of protein and DNA, based on which we then calculated Kd from the probabilities of the bound and unbound states (35) (see more details about the calibration in SI Appendix, Supplementary Methods). The PWM data for Sox2 and Oct4 was downloaded from the JASPAR database (60).

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Let’s start with the basics, shall we? An RV tankless water heater is exactly as it sounds: a water heater that doesn’t use a tank and is compatible with—or specially created for—an RV.

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As portable systems aren’t vented to the outside, it’s perilous to use these inside the RV. As a result, you’ll have to carry the system outside to use it safely in the open air every time you need to shower. Most people purchase a separate tent ensuite to ward off prying eyes.

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Note that electric heaters are more expensive upfront but cost less to run (you’ll be using them off your solar/battery system or the pre-paid shore power at the RV park). Gas heaters are cheaper to buy, but you’ll have to keep topping up your propane tanks, which requires money and effort.

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RV enthusiasts who don’t want to rely on their 120V or 12V system to spark up the water heater might consider the 101 from EZ, which uses 2 D cell batteries to ignite the propane. That way, if there’s a blackout or if cloudy weather renders your solar panels ineffective, you’ve still got the chance to enjoy a nice hot shower to finish off the day.

Electric tankless water heaters are a viable option as they’re eco-friendly and cost-effective to run. The heaters work on the 120V system of your RV, so if you’re planning to use your water heater off-grid, you’ll need to ensure your converter is capable of handling the required wattage. You’ll need an expensive solar and battery set up to reap the benefits of an electric tankless water heater off the grid, but the expense is well worth the reward for van-lifers and full-time RVers. If you’ve installed a full electric system, it’s worth it to take a look at electric trailer jacks as well.

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As a ballpark figure, RV tankless water heaters typically range between 30,000 and 50,000 BTUs. Although some portable models are rated higher, their lower flow rates equate to inferior performance overall.

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What if I told you that you could incorporate the best of both energy sources into the one high-tech design? Well, you can, and they’re colloquially known as combo RV tankless water heaters. These systems are the ultimate RV solution as they can draw on both your gas and electricity supply to give you a wonderfully warm shower whether you’re on or off the grid.

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Another nifty plus is its high-tech LED display, which looks sleek and state-of-the-art while hanging in your ensuite wall. Automatic temperature controls ensure the water never gets too hot, and a child-lock system stops the little ones from messing with the settings.

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If the barista consumes coffee more and brewing more per day, it is recommended to use a coffee drip kettle with a capacity of 1000 mL or larger. If you usually only need 1 or 2 cups, then a 500 mL or 600 ml kettle is sufficient.

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The kettle is very easy to operate and can reach the optimum temperature for making coffee for people. The overall design of the interior and exterior is made of superior stainless steel, so it will not easily rust.

Best Overall RV Tankless Water Heater: Girard 2GWHAM

Portable water heaters aren’t explicitly designed for RV use, although they can indeed be used by folks traveling in a mobile home. The system attaches directly to a propane tank, so it doesn’t require installation and can be lugged around for use anywhere in the great outdoors.

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We’ve covered the top 10 RV tankless water systems available on the market, with various options to suit different budgets and usage requirements. Take a gander through our list to pinpoint the ideal hot water solution for you.

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Portable water heaters are a blessing for folks with small RVs who just don’t have the room to install a fixed shower in their rig. The L10 from Eccotemp is the best of the bunch, providing a nice warm shower sans the time-consuming, permanent setup.

One of the big drawbacks to electric heaters is that their metallic coil heating elements are prone to corrosion and will eventually need replacement. No matter how well you look after your system, limescale and calcium deposits will accumulate and eventually ruin the heating coil. On top of that, the fact that fixing the heater isn’t particularly user-friendly means forking out for a professional repairman on top of the replacement part can cost you.

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With a wattage rating between 7200 and 9600W, the unit is far too power-hungry to use off-grid, so you’ll need to be prepared to shower in the RV park. But if you’re the kind of RVer who spends most of the time connected to the mains, it’s a simple and easy solution for everlasting long showers. Unlike propane, you’ll never have to refill the tank, making it a no-fuss solution for anyone living in an RV park long-term. And at under $200 a pop, going electric is the cheapest way to getting consistent hot water when you need it.

Eccotemp produces some of the finest portable tankless water heaters on the market, earning a reputation for excellence among the outdoorsy camping crowd. Although their fixed hot water systems aren’t quite as highly lauded, this i12-LP model does a respectable job at creating quick hot water without chewing through too much propane.

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Rather than waiting around for the whole tank to warm, tankless water heaters heat the liquid as it passes through the system via a pump, thus allowing hot water to arrive at the faucet in an instant. When you’re cold and want to jump into a soothing hot shower straight away, it’s a godsend to have a tankless water heater ready to roll in your rig.